Membrane Estrogen Receptor Alpha Targeting and its Association with SHC in Regulating Breast Cancer Cell Proliferation
Abstract
Estrogen receptor a, (ERa), the dominant isoform in mammary gland, is a dual functional protein capable of acting as a transcriptional factor regulating gene transcription in the nucleus (genomic effect), or a signaling molecule on the membrane mediating estrogen rapid actions (non-genomic effect) to activate MAPK and Akt pathways. Currently, the functions of the nuclear ERa is very well studied, but the biological role of the membrane-associated ERa is still not clear due to the fact that all ERa-expressing cells have both nuclear and membrane ones co-expressed. One way to investigate the biological role of the membrane-associated ERa in breast cancer cells is to develop a cell line that only express the ERa on the cell membrane or in the cytosol (Designer cells). The goal of this proposal is to develop designer COS-l cells, which lack endogenous ERa, with constructs that express forms of the ERa that are specifically cytosolic or membrane-associated, and use these tools in experiments designed to elucidate the functional role(s) of the membrane-associated, or cytosol ERa, especially its role in interacting and activating Shc adapter protein, leading to MAP kinase activation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2004
- Accession Number
- ADA433404
Entities
People
- Robert X-d. Song
Organizations
- University of North Carolina at Charlotte