Rapid Protein Separations in Microfluidic Devices

Abstract

This paper describes fabrication of glass and plastic microfluidic devices for protein separations. Although the long-term goal is to develop a microfluidic device for two-dimensional gel electrophoresis, this paper focuses on the first dimension-isoelectric focusing (IEF). A laser-induced fluorescence (LIF) imaging system has been built for imaging an entire channel in an IEF device. The whole-channel imaging eliminates the need to migrate focused protein bands, which is required if a single-point detector is used. Using the devices and the imaging system, we are able to perform IEF separations of proteins within minutes rather than hours in traditional bench-top instruments.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 2004
Accession Number
ADA433460

Entities

People

  • Alexander V. Stoyanov
  • Carl K. Fredrickson
  • Champak Das
  • Z. H. Fan
  • Zheng Xia

Organizations

  • University of Florida

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Capillary Electrophoresis
  • Charge Coupled Devices
  • Chemical Reactions
  • Chemistry
  • Detection
  • Detectors
  • Electrophoresis
  • Fabrication
  • Fluorescence
  • Gel Electrophoresis
  • Laser Induced Fluorescence
  • Lasers
  • Manufacturing
  • Mass Spectrometry
  • Polymerase Chain Reaction
  • Proteins
  • Two Dimensional

Fields of Study

  • Physics

Readers

  • Image Processing and Computer Vision.
  • Integrated Circuit Design and Technology.
  • Molecular and Cellular Biochemistry

Technology Areas

  • Directed Energy