Identification of a Bacteria Using Phylogenetic Relationships Revealed by MS/MS Sequencing of Tryptic Peptides Derived From Cellular Proteins
Abstract
Genomes of all priority bacterial pathogens for biodefense purposes and more than one hundred other bacteria have been sequenced. In addition four hundred bacterial genome-sequencing projects are in progress. These achievements provide new possibilities for reliable identification of bacteria on a molecular level by retrieving their genomic information. We present a mass spectrometric approach to link these resources with genomic information encoded in bacterial proteins. Amino acid sequencing of tryptic peptides is performed from bacterial protein extracts. The results of searching tandem mass spectra of peptide ions against a comprehensive bacterial proteome database are analyzed using probability based scoring. This is followed by bacterial identification with an algorithm that uses phylogenetic relationships between bacterial species as a part of a hierarchical decision tree process.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2004
- Accession Number
- ADA433481
Entities
People
- A. P. Snyder
- H. Wick
- J. P. Dworzanski
- Liying Li
- R Chen
- S. V. Deshpande