Process Development For Bacteriorhodopsin Expressed In Escherichia Coli
Abstract
The Army is increasingly dependent on computers and electronic tools to achieve high levels of situational awareness on the battlefield (Opportunities in Biotechnology for Future Army Applications, National Research Council, 2001). With silicon based technologies reaching their technological limits, biologically derived or inspired materials such as protein-based memory systems are viable alternate technologies for the future which will increase their usefulness for Army applications. Bacteriorhodopsin, a light activated protein with unique photophysical properties, an excellent candidate which can be exploited for volumetric data storage, provides promising new methodologies for high-speed signal processing and communication, novel neural networks, linear and nonlinear devices and memories, which can revolutionize the Army's strategic communication infrastructure. Large quantities of readily available bacteriorhodopsin would be especially useful for the further developing bio-derived electronic and photonic materials. In this manuscript we describe the optimization of production and purification protocols for bacteriorhodopsin recovery and yield from a recombinant E. coli fermentation system.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2004
- Accession Number
- ADA433488
Entities
People
- Chundakkadu Krishna
- Joseph F. Kragl
- Michael S. Horsmon
- Patricia E. Anderson