Applying Classical Microbial Identification Strategies to Microarray Platforms
Abstract
In the event of a BW attack, rapid and sensitive methods are needed for accurate identification of bacteria to the species level. Current rapid methods for species-specific identification require clean extraction of pure nucleic acid and require amplification of signals starting material by PCR. Significant microbial expertise may be required to evaluate a sample containing microorganisms so that the correct PCR primers can be selected for positive identification before any tests are run. Although PCR is relatively rapid, the applications are limited to assessment of a small community of microbes and are directed to only one or a few genes at a time. In order to adequately test for all potential bacterial pathogens, many tests are required if no apriori knowledge about the suspected agent is known. This increases the time and cost of analysis results in depletion of available test materials. A better approach would be to reduce the cost of analysis without the concomitant loss of information and sample and without increasing analysis time.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2004
- Accession Number
- ADA433528
Entities
People
- H. Ho
- H. Le
- J. Jackman
- M. Theodore
- N. Boggs
- W. Bethea
Organizations
- Johns Hopkins University