Characterization of the Role of JJAZ1 in Human Breast Cancer
Abstract
We hypothesized that the upregulation of the PcG protein JJAZ1 (now known as Suz12) in human breast cancers leads to chromatin modification and subsequent changes in gene expression. It is difficult to identify genes regulated by PcG proteins due to the fact that these proteins do not directly bind to DNA, but rather are recruited via one or more site-specific DNA binding proteins. Our overall Aim was to demonstrate that our recently developed method, which combines chromatin immunoprecipitation and promoter microarray analysis, could be used to identify a large set of genes that are regulated by JJAZ1/Suz12. We proposed to develop an antibody to JJAZ1/Suz12 and then to use that antibody in combination with a technique which we have developed called ChlP-CpG that would allow us to identify the chromosomal sites bound by JJAZl/Suz12 without prior knowledge of its DNA-binding protein partners. We were successful in developing an antibody to Suz12, have demonstrated that the ChlP-CpG assay can identify Suz12 targets in colon cancers, have collected a number of human breast cancer samples, and are currently completing the studies to identify breast cancer-specific JJAZ1/Suz12 target genes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2005
- Accession Number
- ADA433851
Entities
People
- Peggy J. Farnham
Organizations
- University of Wisconsin–Madison