Prostasin's Role in Prostate Cancer
Abstract
The focus of the project for this report period was the establishment of prostate cancer cell lines with inducible expression of prostasin, for which a role as a tumor suppressor has been implicated. Meanwhile, mapping of prostasin signal transduction pathways was underway using a gene expression knock-down approach in the LNCaP Human prostate cancer cell line. A tetracycline-inducible, prostasin expressing DU-145 cell line has been established, this cell line will allow for proper assessment of prostasin's role as an invasion suppressor and a tumor suppressor. Prostasin serine protease and its serine active-site mutant were able to reduce the motility of DU-145 human prostate cancer cells. Prostasin gene silencing by siRNA in the LNCaP human prostate cancer cells resulted in a down-regulation of the cycln-dependent kinase inhibitor, p21CIP1 at the protein level but not the MRNA level, further suggesting a tumor suppressor role for prostasin serine protease. The protein level change of p21CIP1 is a lead for pathway mapping of prostasin signal transduction.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2004
- Accession Number
- ADA433985
Entities
People
- Karl Chai
Organizations
- University of Central Florida