Anti-Cancer Drug Discovery Using Synthetic Lethal Chemogenetic (SLC) Analysis
Abstract
I am developing a novel cell-based small-molecule screening approach that can identify inhibitors of any non-essential protein function through a surrogate synthetic lethal phenotype in the baker's yeast, Saccharomyces cerevisiae. Synthetic lethality (SL) is a form of genetic enhancement in which two mutations are lethal in combination, but the corresponding individual mutants are viable. Thus, a sensitized yeast strain carrying a mutation that is synthetic lethal with a gene of interest will be inviable in the presence of a chemical inhibitor of the target protein.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2004
- Accession Number
- ADA434327
Entities
People
- David S. Bellows
Organizations
- Mount Sinai Hospital, Toronto