Integrated Massively Parallel Arrays of Stochastic Sensors (IMPASS)
Abstract
We report the successful formation of biomimetic polymer membranes approximately 5-6 nm in thickness made from the biomimetic amphiphilic triblock copolymer poly(methyloxazoline)-poly(dimethylsiloxane)-poly(methyloxazoline). We have also successfully inserted a number of membrane proteins (OmpG, MscL, alpha-hemolysin, and alamethicin) into these membranes and demonstrated that these proteins retain their natural function and capabilities. We have found that these membranes exhibit extended lifetimes compared to lipid membranes and on micromachined substrates the lifetime is extended even further (approximately 3 days). As a result of this work, we have found that biomimetic polymer membranes are suitable subjects for further research in membrane protein-based stochastic sensing.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2005
- Accession Number
- ADA434349
Entities
People
- Carlo Montemagno
- Jacob Schmidt
Organizations
- University of California Regents