Prostatic Fluid Cells
Abstract
Most research that requires long-term propagation of prostate cancer (PCA) cells is carried out with three cell lines: DU 145, PC-3, and LNCaP. All but one of these lines, LNCaP, fail to express prostate-specific antigen (PSA), androgen receptors, and/or any other prostate phenotype. Some prostatic fluids contain prostate cancer cells. The goals of this research were as follows: (1) to test the tumorigenicity of, and to develop transplantable xenografts from, PCA cells in prostatic fluid; (2) to develop methods for enhancing the tumorigenicity of small numbers of these PCA cells without deliberately altering their genes; (3) to test these methods for enhancement of tumorigenicity with prostatic fluid cells; and (4) to initiate clinical followup. As detailed in the body of the report, the aims of the research have been limited by exceptionally weak clinical collaboration that has become progressively weaker. The fluids that the author tested earlier were promising, but samples tested in the past year have been quite inadequate. Co-injection of lethally irradiated, growth-factor-producing cells was encouraging in some experiments with some tumors, but results were quite variable in repeated experiments. The inadequacies in fluid samples and the repeatability of experiments are detailed in this report. The author concludes that, prostate cancer cells from prostatic fluids of a significant proportion of patients with primary prostate cancers can survive for a few months in nude mice, and they do not grow rapidly enough to form transplantable xenografts with the technology that is currently available.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2004
- Accession Number
- ADA434468
Entities
People
- Thomas G. Pretlow
Organizations
- Case Western Reserve University