In Vivo p53 Signaling in Breast Epithelial Cells After Oncogenic Stimulus
Abstract
One of the most frequent alterations in breast cancer is deregulation of the p53 tumor suppression signaling pathways. The tumor suppressor p53 is a sequence-specific transcription factor that is activated in response to various cellular stresses. It has been predicted that there are hundreds of consensus p53 binding sites present in the human genome. To date, numerous seminal p53 target genes have been identified, leading to the further elucidation of the role of p53 in tumor suppression. However, only a subset of p53 target genes has been identified to date, and characterization of p53 signaling pathways in their entirety is not yet complete. The goal of this study is to further define and characterize such pathways through the identification of novel genes that are directly regulated by p53. Using chromatin immunoprecipitation followed by a yeast selection system we have isolated over 100 genomic DNA fragments other that contain novel p53 binding sites. The new DNA fragments obtained have been mapped to various regions of the human genome, and putative novel p53 target genes have been identified, validated, and characterization is ongoing. This research will lead to a more complete understanding of p53-regulated signaling pathways in mammary epithelial cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2004
- Accession Number
- ADA434620
Entities
People
- Jamie M. Hearnes
- Jennifer Pietenpol
Organizations
- Vanderbilt University Medical Center