Use of DNA Microarrays to Identify Diagnostic Signature Transcription Profiles for Host Responses to Infectious Agents
Abstract
Most of the likely agents of bio-terrorism have profound effects on the host and, in particular, on the immune and inflammatory responses. For many of these agents, pathogenesis has been studies at the cellular and molecular levels. These studies indicate that each specific organism has distinctive effects on the host immune and inflammatory cells that contributes to the unique clinical characteristics of the disease. These studies largely have focused on how the agent and its toxins and other constituents modulate host cell expression of individual cytokines and other molecules of interest as well as activation pathways. We have proposed a broad-based approach to identify the unique "signatures" of infectious agents using host DNA micro-arrays. Because of the known diverse patterns of host cell interactions with these organisms, examination of the host transcriptional response has enormous potential to allow rapid diagnosis of infectious diseases in general and agents of bio-terrorism in particular.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2004
- Accession Number
- ADA434780
Entities
People
- Jerrold J. Ellner