The Role of AKT in Androgen-Independent Progression of Human Prostate Cancer
Abstract
To define the causative role of the active Akt in androgen-independent progression of prostate cancer, an inducible Akt (iAKT) is used in this proposal. In this second-year period, androgen-dependent human prostate cancer LNCaP cells stably transfected with vectors carrying iAKT system or the control vectors (empty and kinase-dead mutant iAKTkm) were used to determine if active Akt can protect cell death or promote cell proliferation after serum starvation. We demonstrated that CID-mediated activation of iAKT promotes cell survival only but not cell proliferation of prostate cancer LNCaP cells after androgen or serum withdrawal. We also demonstrated that the established LNCaP.iAKT cells formed xenograft in nude mice and CID) AP22783 injection showed a promising ability to activate the iAKT, and the activated iAKT is functionally capable to phosphorylate the downstream target GSK-3. In the next year, we will use those LNCaP subline cells to determine whether CID-mediated iAKT activation promote tumor growth in castrated nude mice.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2005
- Accession Number
- ADA435055
Entities
People
- Benyi Li
Organizations
- University of Kansas Medical Center