Silk-Elastinlike Copolymers for Breast Cancer Gene Therapy
Abstract
The overall purpose of the project is to use silkelastin-like polymers (SELPs) for the development of controlled gene delivery systems for localized breast cancer gene therapy. The rationale is that by controlling the structure of the polymer, it is possible to control DNA release, duration of transgene expression and the corresponding reduction in tumor size. In year 2 progress was made in the following areas: i) Finished the biosynthesis of three SELP 415K analogs with incremental increase in molecular weight and started on the biosynthesis of SELP 815K, ii) Compared the physicochemical characteristics of hydrogels made from SELP 415K and SELP 47K, iii) Compared the DNA release characteristics of hydrogels made from SELP 415K and SELP 47K and evaluated the interaction of DNA with these polymers. The next logical steps are to finish the biosynthesis of the third analog (815K), conduct characterization and release studies using hydrogels made from this analog and evaluate the delivery systems in murine models of breast cancer as proposed in the application.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2005
- Accession Number
- ADA435058
Entities
People
- Hamid Ghandehari
Organizations
- University of Maryland, Baltimore