Polyphosphate Affects on Breast Cancer Cell Survival
Abstract
Goals met during the first funding period of this grant include developing methodologies to measure cellular polyphosphosphate concentrations in breast cancer cells and the preparation of breast cancer cell lines with altered levels of polyphosphate. Specifically, a reproducible and quantitative procedure to extract and enzymatically measure polyphosphate concentrations in breast cancer cells is now in hand. Additionally, a plasmid containing a copy of the S. cerevisiae exopolyphosphatase (scPPX) gene behind a constitutively active CMV promoter was constructed and successfully stably transfected into breast cancer cell lines. scPPX degrades polyphosphate by processively removing the terminal phosphate moiety from the polyphosphate polymer. Thus, the presence of extra copies of scPPX results in depleted levels of cellular polyphosphate. These accomplishments of the first funding period provide the necessary tools to investigate the link between cellular polyphosphates and breast cancer, as outlined in the proposal.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2005
- Accession Number
- ADA435268
Entities
People
- Christine L. Haakenson
Organizations
- Georgetown University