Role of Reactive Stroma in Prostate Cancer Progression
Abstract
The purpose of this project is to determine the role of FGF receptor 1 in reactive stroma during prostate tumorigenesis. We are using a novel approach to target transgene expression specifically to the reactive stroma of experimental prostate cancer. We are placing an inducible Ore recombinase into the FAP gene locus to target expression to reactive stroma. We will cross this mouse with Fgfr1flox mice (LoxP sites flanking FOF receptor 1 alleles). These mice will be crossed with TRAMP mice (prostate cancer model). Induced expression of Ore at sites of reactive stroma generated in the cancer foci will function to excise the FCF receptor 1 alleles and create a conditional knockout mouse. Progression of tumorigenesis in this line of knockout mice will be compared to heterozygous and wild type controls. We have acquired all clones and reagents and have completed rederiving the Fgfrlflox by embryo transfer. We have nearly completed generating and verifying the constructs necessary to target Ore and the five generations of breeding necessary to obtain the desired genetic background of mice. This study will pinpoint the role of FGF receptor in prostate cancer progression and will help in designing a targeted therapeutic to cancer reactive stroma.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2005
- Accession Number
- ADA435269
Entities
People
- David R. Rowley
Organizations
- Baylor College of Medicine