Cytolytic T Lymphocytes in Organotypic Breast Carcinoma Culture
Abstract
In the proposed studies, novel T cell immunotherapies against breast cancer will be developed based on studies demonstrating a positive correlation between T lymphocytic infiltration of these tumors and a favorable clinical outcome. The major goal of the proposed studies is to isolate and characterize cytolytic T lymphocytes (CTL) with in vivo-like T cell receptors. The CTL provide the basis for adoptive CTL immunotherapy and active immunotherapy with CTL-derived peptides/antigens. During the past 3 months of study approved for inclusion of human subjects, 4 breast carcinoma tissues were cultured in organotypic cultures (reconstructs) and mixed lymphocyte/tumor cultures (MLTC). Eleven T cell lines were obtained from 2 breast cancer specimens and 3 fibroblast cell lines from 3 specimens. The preliminary studies also have shown that breast tumor cells grow in vitro (reconstruct and MLTC), although it is too early to determine the success rate for establishing long-term tumor cell lines. These preliminary studies demonstrate the feasibility of establishing T cell lines against breast cancer cells in a novel culture system with in vivo relevance (reconstruct).
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2004
- Accession Number
- ADA435293
Entities
People
- Dorothee Herlyn
Organizations
- Wistar Institute