INT6 is a Potential Tumor Suppressor for Breast Cancer
Abstract
This project tests the hypothesis that Int6 regulates the 26s proteasome and acts as a tumor suppressor for breast cancer. The authors have accomplished a key step in this study by generating siRNA sequences that knock down Int6 expression, thus influencing mitosis. They also have prepared a good Int6 antibody and will soon learn whether Int6 associates with the proteasome biochemically. They have made great strides in exploring additional angels to better study Int6 functions. First, they have evidence supporting an intriguing possibility that Int6 may be C-terminally truncated in human breast cancer cell lines. Second, their previous studies of the fission yeast system suggest that the tumorigenesis potential induced by Int6 inactivation can be counteracted by oncogenic Ras. They have further investigated the relationship between Int6 and Ras and found that in fission yeast Int6 can co-localize with the Ras-Cdc42 pathway in the endomembrane. They will determine whether this occurs in human cells and ascertain its role in tumorigenesis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2005
- Accession Number
- ADA435295
Entities
People
- Eric Chang
Organizations
- Baylor College of Medicine