Comparison of Demineralized Dentin and Demineralized Freeze Dried Bone as Carriers for Enamel Matrix Proteins in a Rat Critical Size Defect
Abstract
This study evaluated the ability of Emdogain (EMD) in combination with demineralized freeze dried bone (DFDBA) or demineralized dentin matrix (DDM) to enhance bone regeneration as well as the ability of these carriers to maintain EMD at the surgical site. Critical size defects were created in rat calvaria. In Part I of the experiments, varying amounts of EMD (1 mg, 3 mg, 9 mg) with DFDBA or DDM were placed in the defects. DFDBA, DDM, EMD alone, and propylene glycol served as controls (8 rats/group). Rats were euthanized at two and eight weeks. Histological analysis was used to score for the presence of carrier material and new bone formation. The data were analyzed using RIDIT analysis (p%<O.O5). In Part II of the experiments, EMD was labeled with 1251 and placed in the defect alone or combined with DFDBA or DDM particles. Images were acquired using gamma scintigraphy at 0, 4 hour, 1, 2, 3, 6, 13, 20 days. The images were quantified using computer software to determine the retention of EMD in the critical size defect.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2005
- Accession Number
- ADA435301
Entities
People
- Kerri Font
Organizations
- Air Force Institute of Technology