Critical Contribution of Ral GTPases to Growth and Survival of Breast Cancer
Abstract
We have previously identified the Ras-like GTpases, RalA and RalB as linchpin modulators of human tumor cell proliferation and survival. Using RNAi-mediated loss-of-function analysis, we find that RalA is critical for anchorage-independent growth whereas RalB is required for tumor cell survival. The collaboration of these isoform-specific contributions generates a minimal oncogenic platform, which results in aberrant proliferation coupled with suppression of apoptosis. We have begun to characterize the molecular basis of the collaborative contributions of RalA and RalB to cell regulation. These studies have surprisingly revealed that RalB and Sec5 are mediating tumor cell survival whereas RalA and STAT3 contribute to anchorage-independent proliferation. Understanding how the regulation occurs will be the focus of my future work.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2005
- Accession Number
- ADA435549
Entities
People
- Yuchen Chien
Organizations
- University of Texas Southwestern Medical Center