Molecular Mechanisms of Hormone-Refractory Prostate Cancer

Abstract

The concept to be tested herein is that the various tyrosine kinase pathways known to contribute to anti-androgen resistance likely converge on a common signaling molecule that integrates the upstream signaling input and leads to androgen receptor activation in an androgen-independent manner. Identification of the signal-integrating molecule(s) would provide a valuable therapeutic target for prostate cancer. We hypothesized that the intracellular docking protein Cas, complexed with the adapter protein Crk, could have such a signal-integrating role. Our studies performed so far support a role for Crk in androgen resistance signaling pathways. At the same time, our studies suggest that a docking molecule other than Cas, but possessing a similar molecular weight, functions to couple Crk to the upstream tyrosine kinases. Thus, we have established a correlation between androgen independence and Ork signaling, as proposed in the aim 1 of our application. Our next objective is to molecularly identify the signal integrating docking molecule, and then proceed to aim 2 and examine whether activation of Crk signaling is causal to androgen independence in prostate cancer cells in vitro. Our long-term objective is to utilize the obtained information to develop specific and sensitive tools for diagnosis and therapeutics of anti-androgen resistance.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 2005
Accession Number
ADA435559

Entities

People

  • Kristiina Vuori

Organizations

  • Sanford Burnham Prebys Medical Discovery Institute

Tags

DTIC Thesaurus Topics

  • Albumins
  • Androgen Receptors
  • Blood
  • Cell Movement
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Cytoskeleton
  • Molecular Weight
  • Molecules
  • Neoplasms
  • Prostate Cancer
  • Proteins
  • Proteomics
  • Therapy
  • Tyrosine

Fields of Study

  • Biology
  • Chemistry

Readers

  • Breast cancer cell signaling and growth regulation.
  • Prostate Cancer Biology.