Inhibition of Androgen-Independent Growth of Prostate Cancer by siRNA-Mediated Androgen Receptor Gene Silencing

Abstract

To develop a novel therapeutic approach for hormone-refractory prostate cancers, we proposed to knock down human androgen receptor (AR) gene using an RNAi-based technique. Based on our previous work, we generated a recombinant adeno-associated virus bearing a hairpin-structured small interfering RNA against the AR (ARHP8) for long-term expression of the AR siRNA. A control virus bearing the GFP gene only was also produced. We demonstrated that the resultant rAAV.ARHP8 knocked down the AR expression both in protein and mRNA levels in prostate cancer LNCaP cells. Next, we will use the virus to treat prostate cancer xenografts in a nude mice model.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 2005
Accession Number
ADA435609

Entities

People

  • Benyi Li

Organizations

  • University of Kansas Medical Center

Tags

DTIC Thesaurus Topics

  • Androgen Receptors
  • Antisense Elements (Genetics)
  • Apoptosis
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Culture Techniques
  • Cultured Cells
  • Epithelial Cells
  • Gene Expression
  • Membrane Potentials
  • Neoplasms
  • Programmed Cell Death
  • Prostate Cancer
  • Proteins
  • Therapy

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Prostate Cancer Biology.