Genome-Wide Nucleic Acid/Protein Interaction in Breast Cancer
Abstract
Since many types of breast cancer remain untreatable, the research proposal aims to develop novel genomic technology to identify potential therapeutic targets and to aid in diagnosing various types of breast cancer at the molecular level. The overarching goal of the proposal is to develop a technology to screen nucleic-acid protein interactions on a genome scale with a focus on understanding complexes involved in breast cancer. In order to identify the regulatory networks of interactions between RNAs and proteins, we proposed to develop a rapid genome-scale method to determine the specific RNA targets and RNA binding sites of proteins. The aims were to 1) discover RNA targets of specific RNA binding proteins and 2) define the RNA sequences recognized by proteins using novel nanotechnologies including development of optically encoded beads containing both a unique optical signature and a specific oligonucleotide. This technology is being complemented by genome-wide chromatin immunoprecipitation and RNA profiling on exon arrays. Progress during the past year has been made on Aims 2, 3 and 4 of the original grant as detailed below. Two papers mapping proteins to the genome have been submitted for publication while a third paper is in preparation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2005
- Accession Number
- ADA435620
Entities
People
- Pamela Silver
Organizations
- Dana–Farber Cancer Institute