Testing of the Bio-Seeq (Smiths Detection Handheld PCR Instrument): Sensitivity, Specificity, and Effect of Interferents on Yersinia Pestis Assay Performance
Abstract
Smiths Detection-Edgewood (SDE), Inc., recently developed a handheld PCR instrument, the Bio-Seeq(Federal Registration), an updated, redesigned version of a small, portable PCR instrument previously known as Handheld Advanced Nucleic Acid Analyzer (HANAA). The SDE developed the Bio-Seeq(Federal Registration) to provide a portable platform for use by first responders to detect biological threats in civilian areas. To be ready for introduction into the marketplace, the instrument must be accompanied by a menu of reagents that will enable the user to detect the presence of pathogens in environmental samples. In previous work, SDE obtained probe and primer sequences constituting an assay for a gene present in Yersinia pestis, the causative agent of plague. The SDE incorporated this probe and primer set into dried reagent beads, which also contain reagents required for an internal control. These, in turn, are part of a self-contained sampling device that contains buffer and the PCR reagent beads. This report details an analysis of the assay for Y. pestis, including the sensitivity and specificity of the assay, and the effect of some common non-target (interferent) materials on the performance of the assay.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2005
- Accession Number
- ADA435765
Entities
People
- James J. Valdes
- Jennifer R. Bucher
- Kevin P. O'connell
- Patricia E. Anderson
Organizations
- Edgewood Chemical Biological Center