IGF-Regulated Genes in Prostate Cancer
Abstract
We hypothesized that genes that are differentially expressed as a result of the decreased IGF-I receptor gene expression seen in metastatic prostate cancer contribute to prostate cancer progression, and include metastasis-regulating genes that could constitute valuable diagnostic markers or therapeutic targets. We initially proposed three specific aims: 1) Identification of differentially expressed genes in isogenic metastatic vs. non-metastatic prostate epithelial cells; 2) Identification of proteins that are differentially secreted in these cell lines, and 3) Assessment of the differential expression of these genes and proteins in laser-microdissected samples. We have used microarray gene profiling to characterize differentially expressed genes and have used SELDI-TOF mass spectrometry to identity proteins that are differentially secreted into conditioned media. In the last year, we obtained initial data suggesting that elevated IGF-I receptor expression controlled survival in adult human male serum, which may explain the relationship between IGF action and metastasis. We proposed to take advantage of these new findings by investigating the molecular mechanisms underlying this effect and the cell-surface molecules expressed in metastatic and non-metastatic cells responsible for the differential sensitivity to serum, and requested a change in the statement of work. Unfortunately, these findings were not repeatable with subsequent batches of human serum, so that we have obtained a no-cost extension through 2/06 to readdress the marginal specific aims.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2005
- Accession Number
- ADA435794
Entities
People
- Charles T. Roberts Jr.
Organizations
- Oregon Health & Science University