Investigating the Role of FIP200 in Mammary Carcinogenesis Using a Transgenic Mouse Model
Abstract
TRE2-HA-FIP2OO-IRES2-EGFP-h beta g3' DNA fragment was used to create transgenic mice by pronuclear injection into fertilized FVB/N eggs. Three independent TRE2-FIP2OO founders were identified with PCR screening with EGFP primers. All three founders and their transgenic offspring are clinically normal, fertile, their litters are of normal size, their pups exhibit normal growth-rate. Mammary gland morphology is normal in all physiological stages in the TRE2-FIP2OO mice when compared to wild-type littermates. Double transgenic offspring were created by mating TRE2-FIP2OO transgenic mice to the regulator (MMTV-rtTA) mice. The double transgeninc mice are clinically normal, fertile, their litters are of normal size, their pups exhibit normal growth-rate. After doxycycline supplementation of the drinking water the transgenic FIP2OO message can be detected with RT-PCR in one line (c3) of double transgenic animals. So far, attempts to detect transgenic FIP2OO protein with immunoblotting, immunoprecipitation, and immunohistochemistry were unsuccessful. Experiments are planned to solve this problem. Experiments are being conducted to monitor mammary gland development in the double transgenic animals during pregnancy with or without doxycycline treatment.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2005
- Accession Number
- ADA435823
Entities
People
- Tamas Nagy
Organizations
- Cornell University