A Functional Genomic Analysis of NF1-Associated Learning Disabilities
Abstract
Learning disabilities severely deteriorate the life of many NF1 children by limiting their academic achievement, higher education and career choice. However, the pathogenic process for NF1-associated learning disabilities has not been fully understood and an effective +1- therapy is not available. This study was proposed to identify genes that are dysregulated in the hippocampus of the NF1 mouse model by DNA microarray analysis. Characterization of these NF1-affected genes will dramatically improve our understanding of the molecular pathogenesis underlying NF1-associated learning deficits. During the first year of the project, we have (i) established an NF1 mouse colony to generate NF1 mice used in this study, (ii) purified RNAs from the NF1+/- and wild-type hippocampus and (iii)performed LTP experiments on NF1+/- and wild-type hippocampal slices to prepare slices after LTP induction for RNA purifications. These progresses lay down a firm foundation for us to complete the proposed microarray experiments in the next two years.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2005
- Accession Number
- ADA435850
Entities
People
- Shao-jun Tang
Organizations
- University of California, Irvine