Development of a Novel Tissue slice Culture Model of Human Prostate Cancer

Abstract

This project achieved all of its goals. Preparation of prostate tissue slices was optimized by a variety of technological improvements. Optimal culture conditions were identified for the maintenance of structure and function of cultured tissue slices for at least 48 hours. The requirement for androgen to maintain the secretory epithelium in cultured tissue slices was demonstrated. The effects of a number of clinically relevant compounds on gene expression, protein expression, structure and function were effectively analyzed in tissue slice cultures. We conclude that challenges still remain to achieve long-term culture of tissue slices with full preservation of structure and function, but unique preclinical information can already be gained from this methodology as it has been developed so far.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 2005
Accession Number
ADA435857

Entities

People

  • Donna M. Peehl

Organizations

  • Stanford University

Tags

DTIC Thesaurus Topics

  • Androgens
  • Apoptosis
  • Cells
  • Culture Techniques
  • Cultured Cells
  • Epithelial Cells
  • Epithelium
  • Filter Paper
  • Gene Expression
  • Maintenance
  • Pilot Studies
  • Polymerase Chain Reaction
  • Prostate
  • Prostate Cancer
  • Proteins
  • Tissues
  • Vitamin D

Readers

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