Analysis of Rat Blood Samples for Agent Biomarkers After GB Inhalation Exposure
Abstract
A method was developed for the analysis of a GB nerve agent biomarker in blood that is very sensitive, selective, and applicable for archived samples. The biomarker resulting from sample acidification and in the presence of fluoride ion was regenerated GB (rGB) which was found in rat blood after inhalation exposures ranging from miosis to lethal levels. The method utilized a C18 solid-phase extraction (SPE) followed by quantification using a gas chromatograph with either a flame photometric detector (GC-FPD) or a mass spectrometer (GC-MS). Samples were concentrated by injecting the SPE extract on a Tenax -TA sorbent tube along with 100 pg of decadeuterated diethyl ethyl phosphonate as the internal standard followed by thermal desorption GC-FPD analysis and GC-MS confirmation. The method detection limit was 6 pg of agent and the working range was 20-200 pg GB on column. Quality control samples were analyzed and yielded spike recoveries greater than 95%.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2002
- Accession Number
- ADA436016
Entities
People
- B. Gaviola
- C. Crouse
- D. C. Burnett
- E. M. Jakubowski
- H. Dupont Durst
- J. Anthony
- J. Manthei
- L. S. Heykamp
- R. J. Mioduszewski
- R. Way
- S. A. Thomson
- W. Muse
Organizations
- Edgewood Chemical Biological Center