Studies in the Formation of Ethyl Methylphosphonofluoridate From Rat and Human Serum Exposed to VX and in the Presence of Fluoride Ion
Abstract
A method has been developed for the analysis of a VX nerve agent biomarker in blood that is very sensitive and selective. VX was detected in spiked human and rat sera by the generation of its corresponding G-series derivative, ethyl methyl phosphonofluoridate (VX-G). This method utilizes a C18 solid-phase extraction (SPE) followed by quantification using a gas chromatograph with either a flame photometric detector (GC-FPD) or a mass spectrometer (GC-MS). VX-G was completely resolved from sarin (GB) and the method has the potential to resolve other nerve agents in the VX series of cholinesterase inhibitors. The method detection limit was 10.5 pg of agent on column.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2002
- Accession Number
- ADA436017
Entities
People
- E. M. Jakubowski
- H. Dupont Durst
- L. S. Heykamp
- S. A. Thomson
Organizations
- Edgewood Chemical Biological Center