Modeling Human Epithelial Ovarian Cancer in Mice by Alteration of Expression of the BRCA1 and/or P53 Genes
Abstract
About 1 out of every 10 cases of epithelial ovarian cancer is inherited. Unlike non-hereditary (sporadic) ovarian cancer, some of the underlying genetic causes of hereditary ovarian cancer are well understood. The majority, more than 90%, of inherited cases are the result of inherited mutations in the breast cancer associated gene 1 (BRCA1). In addition to mutations of BRCA1, mutations of the p53 gene are often found in patients with breast and ovarian cancer syndrome. Based on the importance of both of these genes in the development of this type of ovarian cancer, the authors hypothesize that inactivation of BRCA1 and p53 in the ovaries of mice will result in epithelial ovarian cancer in the animals. They have obtained mouse strains that conditionally express the BRCA1 and p53 genes. Tissue-restricted exposure to cre recombinase results in excision of LoxP flanked (floxed) sequences in these mice and expression of the mutated forms of BRCA1 and p53. To achieve cre recombinase expression that is restricted to the ovary, they have employed localized delivery of Adenovirus-Cre by intrabursal injection of virus. They also have developed transgenic mice that should express cre recombinase in a reproductive tissue-restricted and drug inducible manner.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2005
- Accession Number
- ADA436423
Entities
People
- Denise C. Connolly
Organizations
- Fox Chase Cancer Center