Using Phage Lytic Enzymes to Destroy Pathogenic and BW Bacteria
Abstract
We have exploited the rapid, lethal and highly specific action of bacteriophage lytic enzymes to destroy pathogenic bacteria. Our results show that in vitro 10(7) bacteria can be reduced to sterility seconds after enzyme contact. We now have enzymes that are specific for S. pyogenes, S. pneumoniae, and B. anthracis S. aureus, E. faecalis/E. faecium and group B streptococci. In animal models, we pre-colonize mice with either streptococcal or pneumococcal species (orally or nasally) and remove them completely with a single dose of phage enzyme delivered to these sites. In a septicemia model with S. pneumoniae, bacteria are reduced by >2-logs from the blood of infected animals with a single intravenous dose of enzyme. A lytic enzyme called PlyG from the gamma-phage of B. anthracis was specific for all worldwide isolates of B. anthracis. When >1 LD1 00 of B. anthracis bacilli were delivered i.v. to mice only 10% of animals, followed for 12 days, survived. When PlyG was injected i.v. 15 min after infection, 90% of the mice recovered fully. Resistance to the enzymes has not been found nor do antibodies neutralize their activity. Thus, phage lytic enzymes are a new reagent to control resistant pathogenic bacteria, offering a capability previously unavailable.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 14, 2005
- Accession Number
- ADA436735
Entities
People
- Vincent A. Fischetti
Organizations
- The Rockefeller University