Combined Biology and Bioinformatics Approaches to Breast Cancer
Abstract
We identified a novel transcription factor LMO4 and found that LMO4 is highly expressed in breast epithelial cells and is related to cell proliferation and/or invasion in vivo. Because these cellular features are associated with breast carcinogenesis and because LMO4 is overexpressed in more than 50% of breast cancer cases, we hypothesize that LMO4 may play roles in oncogenesis of breast epithelial cells by regulating proliferation, invasion and/or other cellular features. As the initial step of the whole projects, we established several permanent cell line (including breast normal epithelial cells and cancer cells), which stably overexpress LMO4 protein or express LMO4 RNAi to delete LNO4 protein. Using these cell lines. We will analyze the biological effects of LMO4 on cell proliferation, invasion and other cellular features in vitro, and screen the target gene of LMO4 by gene microarray. We have obtained some exciting results and found that LMO4 modulates TGF-beta signaling, which plays the crucial roles in both development and cancer. Our results show LMO4 associated with TGF-beta signaling target gene promoter by interacting with Smad proteins, and enhanced the inhibitory effect of TGF-beta on cell proliferation of mammary gland epithelial cells. These results strengthen the hypothesis that LMO4 may contribute to the oncogenesis of breast tissue and indicates that our whole work will play a role in solving the breast cancer problem on the support of the Army.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2005
- Accession Number
- ADA436927
Entities
People
- Zhongxian Lu
Organizations
- University of California, Irvine