An Oral DNA Vaccine Encoding Endoglin Eradicates Breast Tumors by Blocking Their Blood Supply

Abstract

Among novel strategies preventing or slowing breast cancer metastasis are those blocking continuous new blood vessel growth, i.e. angiogenesis in the tumor vasculature mediated by proliferating endothelial cells lining such vessels. These cells overexpress a glycoprotein called endoglin which stimulates such vessels. We successfully constructed and evaluated an oral endoglin-based DNA vaccine and demonstrated its capability to induce a robust CD8(+) T cell response that specifically killed proliferating endothelial cells in the breast tumor vasculature. This, in turn, was shown to markedly decrease tumor angiogenesis resulting in a decisive suppress ion of breast tumor metastases leading to doubling in life-span of successfully vaccinated mice in a prophylactic breast tumor metastasis model.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2005
Accession Number
ADA437032

Entities

People

  • Ralph A. Reisfeld

Organizations

  • Scripps Research

Tags

DTIC Thesaurus Topics

  • Angiogenesis
  • Blood
  • Blood Vessels
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cells
  • Coding
  • Diseases And Disorders
  • Endothelial Cells
  • Epithelial Cells
  • Lymphatic System
  • Lymphocytes
  • Metastasis
  • Neoplasms
  • Proteins
  • Vaccines

Fields of Study

  • Biology
  • Medicine

Readers

  • Immunology
  • Molecular Biology and Genetics

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech