The Role of the Y-Located TSPY Gene in Prostatic Oncogenesis
Abstract
The TSPY gene is the only functional gene within the critical region harboring the gonadoblastoma locus on the Y chromosome (GBY). Expression studies demonstrated that it is aberrantly expressed in prostate cancer. This project is designed to address the role of this putative oncogene on the Y chromosome in this male-specific cancer. The objectives are: l) to identify the oncogenic or tumor promoting domain in TSPY, and 2) to correlate TSPY over-expression with prostatic oncogenesis in transgenic mice. We have analyzed the expression of TSPY in additional cases of gonadoblastoma and samples from a tissue recombination model of human prostate cancer. Our results, so far, provide a detailed morphologic evolution of the oncogenic process in gonadoblastoma and testicular germ cell tumors and demonstrated that TSPY is co-expressed with several known germ tumor markers. We also detect TSPY expression in a hormone-induced prostate cancer model in Noble rat. Over-expression of TSPY potentiates cell proliferation. We attribute such phenomenon to a shortening of the G2/M stage of the cell cycle. We show that TSPY might exert such an effect by binding to type B cyclins. The present research provides significant insights into the probable mechanism of TSPY function in oncogenesis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2005
- Accession Number
- ADA437099
Entities
People
- Yun-fai C. Lau
Organizations
- Northern California Institute for Research and Education