Strategic for Treatment of Bone Metastases from Breast Cancer
Abstract
The major goal of this project is to optimize the strategies for using radioactive strontium for palliation of bone pain due to breast cancer metastases. One approach is to determine ways of selectively increasing strontium uptake at the metastatic site. Estrogens and a variety of other pharmacological agents can influence cellular uptake of calcium and strontium. Cultures of hormone sensitive and hormone independent breast cancer cells and fetal steoblasts and osteoclasts are being examined for their ability to accumulate strontium. Therefore we have standardized the method for estimating strontium in cell extracts using atomic absorption spectrometry. Bisphosphonates are useful in the treatment of osteoporosis and bone metastases. Bisphosphonates sequester calcium and strontium. Bisphosphonates are charged molecules that do not easily cross hydrophobic cell membranes. The nature of the counter ion to the anionic phosphonate was found to have an effect on the cellular uptake of bisphosphonate such as etidronate. Compounds such as spermidine enhanced the effect of etidronate, presumably by increasing cellular uptake.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2004
- Accession Number
- ADA437147
Entities
People
- Rajagopalan Sridhar
Organizations
- Howard University