Optimization and Characterization of Prostate Cancer Targeting Peptides
Abstract
The goal of the project was to characterize and optimize previously identified peptide ligands targeting receptors expressed on the surface of DU-145H prostate cancer cell line. We performed structure activity study with these peptides and we identified the minimum required sequence for binding as well as the amino acids essential for the interaction with the receptor. The peptide lead structures were used to prepare several secondary libraries containing mostly unnatural building blocks in order to identify ligands with stronger affinity to the DU-145H. The lead peptides were also used to prepare the multimeric ligands and ligands for affinity labeling of cell surface receptors involved in the interaction. The results showed that these peptides can isolate alpha-6 integrin from the cell lysate.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2005
- Accession Number
- ADA437205
Entities
People
- Jan Marik
Organizations
- University of California