Function of Human Selenium-Binding Protein in Selenium Induced Growth Arrest and Apoptosis in Prostate Cancer Cells

Abstract

The proposed study required the creation of prostate cell lines that contain hSP56 transgene and it is regulated artificially. During the first year of this study, preparation of inducible mammalian expression construct of hSP56 gene and anti sense expression construct was accomplished. Prostate cancer cell line PC-3 expresses hSP56 extremely low and they are stably transfected with the above-described expression constructs. Independent clones of PC-3 cells that contain transgene of hSP56 were selected and screened for inducible expression of hSP56 by immunoblotting. Anti-sense R&A method was used to down-regulated the endogenous hSP56 in LNCaP cells and an effective interference RNA was identified for hSP56 gene. The down regulation of hSP56 in LNCaP cells was confirmed by immunoblotting as well.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2004
Accession Number
ADA437732

Entities

People

  • Yuxun Wang

Organizations

  • Beth Israel Deaconess Medical Center

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Biomedical Research
  • Cancer
  • Carrier Proteins
  • Cell Line
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Culture Techniques
  • Gene Expression
  • Neoplasms
  • Organoselenium Compounds
  • Prostate
  • Prostate Cancer
  • Proteins
  • Regulations
  • Selenium

Fields of Study

  • Biology

Readers

  • Cellular and Molecular Pathways of Apoptosis.
  • Oncology (Cancer Research).
  • Prostate Cancer Biology.