Low Melatonin Production During Adulthood - Phase 2: Association with Levels of Hydroxyl Radical Scavenging and DNA Damage
Abstract
The primary purpose of the proposed study is to develop cross-sectional evidence concerning whether or not lower melatonin production levels are associated with increased oxidative DNA guanine damage. If the results of this study are supportive, then confirmatory studies would be warranted, followed by prospective chemoprevention studies of melatonin supplementation. Adjuvant cancer treatment studies have not identified any serious melatonin toxicities. High-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI MS/MS) will be used to simultaneously quantitate urinary levels of 8- hydrodeoxyguanosine (8-oxodG), 8-hydroxyguanosine (8-oxoGuo), and 8-hydroxyguanine (8-oxoGua). This assay appears superior to both the comet and the HPLC-ECD assays. Assays to estimate melatonin production have already been performed in Phase 1. The existing complete overnight urine samples have been properly processed and stored for the proposed study. Fifty-five (55) mother-daughter-father triples of urine samples along with detailed epidemiologic questionnaires are available.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2004
- Accession Number
- ADA437757
Entities
People
- Eugene L. Sobel
- Henrik Poulsen
- Zoreh Davanipour