The Regulation of Nucleolin Expression in Prostrate Epithelial Cells; Possible Involvement of MYC
Abstract
The focus of the two papers that comprise this thesis is on the expression of Nucleolin in prostate epithelial cells. We examine that expression on two levels, mRNA and protein. Our concern in both cases is not so much the actual mechanism by which the two types of molecules are synthesized, but more the regulatory mechanism(s) that allow for their maintenance. In the case of Nucleolin mRNA, we wished to understand what we think is an aberration in its expression profile in a particular instance, that instance being a prostate metastatic cell line that has been serum-starved and then serumrescued. Contrary to results obtained by other research groups, our data show that the mRNA level stays constant throughout this particular experiment. We considered a priori the different reasons to account for this constancy, and we then conducted experiments to determine which scenario offered the best possible explanation. In the case of Nucleolin protein, we unexpectedly discovered a discrepancy between the expression of the mRNA and that of the protein: despite the downregulation of mRNA after serum starvation, the protein remained at the same level exhibited in asynchronously-growing cells; and despite the rise in the mRNA levels during serum rescue of the cells, the protein level showed no corresponding increase. As in our first paper, we thought of possible explanations for this disparity and then attempted to provide experimental justification for one or more of these explanations.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2002
- Accession Number
- ADA437825
Entities
People
- Steven K. Kim
Organizations
- Uniformed Services University of the Health Sciences