Standoff Biological Threat Agent Monitoring and Neutralization
Abstract
SOur objective is to identify fluorescent dyes that can bind to or react quickly with bacterial spores including anthrax spores. B. subtilis spores (which have surface properties similar to that of anthrax spores) were used in screenings against a collection of fluorescent dyes for fast binders with high affinity or for covalent-binders. It is hoped that the attachment of organic molecules to spores will allow rapid standoff detection by acoustic method developed at Los Alamos by Dr. Dipen Sinha. Thirty two dyes were positive in the initial screenings and 14 dyes were confirmed by laser confocal microscopy. Five dyes have been chosen for the next phase of work including the measurement of binding kinetics and affinity constant, the determination of the nature of binding, and, the measurement of shift in fluorescent spectra upon binding.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2003
- Accession Number
- ADA438037
Entities
People
- Lan Bo Chen