Ets2 Regulation of ErbB2-Induced Mammary Tumors
Abstract
Ets2 is a transcription factor that has been implicated in growth factor signaling and is essential for embryonic development. Reduced Ets2 activity restricts the appearance of transgenic mammary tumors caused by either Polyoma middle T antigen (PyMT) or activated Neu/ErbB2. In addition, the early growth of transplanted mammary tumors is limited by host Ets2 activity. Here we have tested genetically, with the use of a conditional Ets2 sub flox allele and tissue specific Cre recombinase expression, whether Ets2 also functions within tumor cells by inactivating Ets2 within mammary luminal epithelial cells from which transgenic PyMT sub Y315/322F tumors arise. We find that inactivation of Ets2 within tumor cells has no effect on tumor appearance or growth. By contrast, complete inactivation of Ets2 in both epithelial and stromal cells moderates the early hyperplastic phase of tumor development and the time of tumor appearance but does not prevent tumor occurrence and has no detectable effect on tumor growth. Thus Ets2 supports mammary tumors exclusively through their microenvironment.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2005
- Accession Number
- ADA438209
Entities
People
- Fang Wen
- John Tynan
Organizations
- Sanford Burnham Prebys Medical Discovery Institute