EGR-1 Functional Activation, Signaling, and Radiation Response in Prostate Cancer
Abstract
In the grant proposal, we hypothesized that in prostate tumors, radiation will up-regulate pro-survival genes and confer an "induced radiation resistance" phenotype. Overexpression of EGR-1 protein will inhibit radiation-induced pro-survival genes that lead to enhanced radiation-induced apoptosis and tumor regression. Two specific aims were proposed: Aim-1: To determine the functional role of EGR-1 overexpression in the inhibition of radiation-induced pro-survival factors such as NFkB and Bcl-2, and its' impact on radiation response, as judged by tumor regression in prostate cancer cell lines xenografts. Aim-2: To determine the relative effect of radiation on the regression of prostate carcinoma in-situ in TRAMP mice versus TRAMP/Egr-1(-/-)mice. Dimova's resignation (prior PI), I was selected to be the PI of this grant. The following tasks were achieved: Aim 1: Two trials were conducted to assess the impact of Adenoviral EGR-1 therapy in combination with radiation. In Trial 2, tumor tissues were collected for molecular signaling analysis. Aim 2: The breeding of EGR-1 knockout mice and TRAMP mice is in progress. We are currently increasing the population of double transgene TRAMP/EGR-14(-/-) for further experiments. Imaging protocol has been developed and regression of prostate tumor in-situ was assessed in few animals.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2005
- Accession Number
- ADA438336
Entities
People
- Mansoor M. Ahmed
- Marianna Z. Sultanov
Organizations
- University of Kentucky