Defining the Regulation of Telomerase Through Identification of Mammary-Specific Telomerase Interacting Proteins

Abstract

Telomerase is a cellular reverse transcriptase that is associated with over 90% of human breast cancers and is composed of 2 integral components, an RNA template (hTR - human Telomerase RNA) and a catalytic polymerase (hTERT - human TElomerase Reverse Transcriptase) (Weinrich et all 997). Telomerase is an obvious chemotherapeutic target (Shay and Bacchetti, 1997). Telomerase activity requires its two core components, hTERT and hTR, to be assembled into a functionally active enzyme by the Hsp9O chaperone complex (Holt et. al., 1999). We have previously demonstrated that chaperones are essential for optimal telomerase assembly in vitro (Holt et. al., 1999) and that Hsp90 itself remains associated with the functional telomerase complex (Forsythe et. al., 2001) (see Figure 1).

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 2005
Accession Number
ADA438428

Entities

People

  • Shawn E. Holt

Organizations

  • Virginia Commonwealth University

Tags

DTIC Thesaurus Topics

  • Breast Cancer
  • Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chromosomes
  • Culture Techniques
  • Department Of Defense
  • Fish
  • Genetics
  • Identification
  • Liquid Chromatography
  • Mass Spectrometry
  • Medical Personnel
  • Neoplasms
  • Proteins
  • Tumor Cell Line

Fields of Study

  • Biology

Readers

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