One-Carbon Metabolism and Methylation in Breast Tumors
Abstract
The first aim of this case control study is to examine the risk of breast cancer in relation to germ-line variants found in three key enzymes related to one-carbon metabolism. We also plan to determine the Methylation status of several genes in our cases and to identify any association with polymorphisms found in enzymes related to one-carbon metabolism. Finally we determined the ER status of all cases in an attempt to identity associations with p53 mutations. In the last year, I have completed SNP data analysis for all (4) polymorphisms and trouble-shoot problems that developed in the methylation assay I have also prepared abstracts poster presentatioas and I m in the process of preparing the first manuscript. We found reduced risk among postmenopausal women in the high folate category associated with MYHFR A1298C. We also found increased risk among postmenopausal women associated with MTHFR C677T, particularly those who consumed inadequate amounts of folate, vitamin B6 and B12, and increased alcohol consumption. Gene-dose effects were noted. MS A2756G was associated with a decreased risk among premenopausal women in the upper B12 category and posnnenopausal women in the lower folate category. This data is supportive of a role for one-carbon metabolism in the etiological link for diet, alcohol drinking and breast cancer sisk.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2005
- Accession Number
- ADA439140
Entities
People
- Peter Shields
- Simone B. Sumner
Organizations
- Georgetown University