Mechanism of Hormonal Regulation of CAD Gene Expression in Human Breast Cancer Cells

Abstract

The CAD gene is trifunctional and expresses carbamoylphosphate synthetase/aspattate casbamyltransferase/ditiydoorotase, wbich are required for pyrimidine biosynthesis. TCDD inhibited E2-induced expression of CAD gene and also downregulated E2-mediated transactivation of CAD gene promoter constructs in transient transfection assays. Moreover the CAD gene promoter construct with a mutant DRE was also inhibited by TCDD suggesting that DRE is not important in Ahr mediated inhibition of CAD gene expression. Using fluorescence resonance energy transfer (FRET), we show that TCDD inhibits the direct interaction between ERa and Spi that is observed in presence of E2.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2005
Accession Number
ADA439222

Entities

People

  • Shaheen Khan

Organizations

  • Texas A&M University

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cells
  • Co-Channel Interference
  • Efficiency
  • Energy
  • Energy Transfer
  • Gene Expression
  • Inhibition
  • Inhibitors
  • Neoplasms
  • Regulations
  • Tissue Extracts
  • Transfection
  • Tumor Cell Line

Readers

  • Breast cancer cell signaling and growth regulation.
  • Molecular Genetics
  • Oncology and Biomarker-Based Cancer Detection.