Novel Micro/Nano Approaches for Glucose Measurement Using pH-Sensitive Hydrogels
Abstract
This project aims to couple environmentally-sensitive hydrogel materials with immobilized enzymes and two novel readout techniques to develop general platforms for biosensing. The need for improved testing methods for diabetics as well as the potential for use of glucose level as a general marker of metabolic status is the primary motivation for this work. This project is focusing on the use of sensitive transduction schemes that allows reliable detection of small physical/mechanical changes in gel size or water content, specifically: microelectromechanical systems (MEMS) and fluorescence resonance energy transfer (RET) optical systems. At the completion of one project year, successful demonstrations of glucose sensing have been accomplished in both areas, and interesting discoveries of hydrogel structure and properties have been uncovered using the novel measurement approaches. While the project is ahead of schedule according to the expected milestones for demonstrating sensing capabilities, optimization of sensitivity, range, and stability are key goals for the second project year. The project has generated three manuscripts to be submitted for peer review and four conference reports, with similar products expected for the second year.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2005
- Accession Number
- ADA439244
Entities
People
- Haifeng Ji
- Mike Mcshane
Organizations
- Louisiana Tech University