Monitoring of Breast Tumor Response to Local Chemotherapeutic Agent Delivered by Biodegradable Fibers
Abstract
For the second year of the project, we have investigated the cyclophosphamide dose effects on rat breast tumor hemodynamics and also monitored how tumor hemodynamics is affected by a vascular disrupting agent by a near-infrared spectroscopy. For experiments, we have applied systemic chemotherapy of cyclophosphamide (CTX) to two groups of rats bearing syngeneic 13762NF mammary adenocarcinomas: one group received a single high dose i. p. (200 mg/kg CTX) and the other group metronomic low doses (20 mg/kg CTX i. p. for 10 days). Combretastatin (CA4P) which is one of vascular disrupting agents was also administered to the rats (30 mg/kg, i. p.) to see its effects in tumor hemodynamics. After CTX treatments, significant changes in vascular hemodynamic response to oxygen inhalation were observed from both groups. The preliminary results suggest that cyclophosphamide has greatest effect on the well perfused tumor vasculature. CA4P administration caused significant decreases in tumor blood volume and oxygenation, and tumor vasculature recovered its function slowly at day 1 after CA4P administration. Overall, our study supports our earlier hypothesis, proving that the effects of chemotherapy in tumor may be monitored non-invasively by using NIRS by detecting the changes of hemodynamics induced with respiratory challenges.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2005
- Accession Number
- ADA439252
Entities
People
- Jae G. Kim
Organizations
- University of Texas at Arlington