Translational Regulation of PTEN/MMAC1 Expression in Prostate Cancer

Abstract

In this project, we proposed to use a dicistronic expression system to determine whether the long 5'-UTR sequence of PTEN contains internal ribosome entry site (IRES) which can mediate cap-independent translation. We have accomplished the proposed work as planed. However, we found that the long 5'-UTR sequence of PTEN does not have the predicted IRES activity but rather contains a strong promoter. We have mapped this promoter and it is likely responsible for constitutive production of the PTEN mRNAs with shorter 5'-UTRs in prostate cancer cells which would be compatible for cap-dependent translation initiation. We have also created a promoterless dicistronic vector and validated its use as a more stringent assay for testing cellular IRES activities.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2005
Accession Number
ADA439271

Entities

People

  • Jian-Ting Zhang

Organizations

  • Indiana University

Tags

DTIC Thesaurus Topics

  • Biological Factors
  • Biomedical And Dental Materials
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Epithelial Cells
  • Genetics
  • Mrna
  • Neoplasms
  • Phosphodiesterases
  • Polymer Chemistry
  • Polymeric Films
  • Prostate Cancer
  • Proteins
  • Ribonucleic Acids
  • Stem Cells

Readers

  • Molecular Genetics
  • Molecular and genetic basis of cancer.