Manipulation of NF-KappaB Activity in the Macrophage Lineage as a Novel Therapeutic Approach
Abstract
The aim of this proposal is to investigate the contribution of NF-kB signaling within macrophages in defining the macrophage's role in normal and neoplastic mammary development. The proposed experiments use multiple murine lines and novel assay systems. We have been developing novel murine models with which to address this issue. We are continuing to use our established IkBa and transplantation technology to investigate the effects of constitutive NF-kB activity within macrophages on mammary development and believe that elevated NF-kB may result in increased macrophage recruitment. To more effectively monitor NF-kB activity within specific cell types we have undergone a collaborative effort and generated a novel Luciferase/GFP reporter transgenic. The inducible model system that we proposed to use in our studies requires three transgenic components; an inducible dominant inhibitor, an inducible constitutive activator and a macrophage specific expressor of the reverse transactivator protein. We have generated and confirmed the functionality of the first two transgenics and are characterizing the third. We are making good progress towards the goal of the study.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2005
- Accession Number
- ADA439787
Entities
People
- Fiona E. Yull
Organizations
- Vanderbilt University